Horse Owner’s Guide to Understanding Equine Blood Testing

Horse Owner’s Guide to Understanding Equine Blood Testing

Why Conduct Blood Tests?

Blood tests are essential for assessing a horse’s overall health, diagnosing diseases, and monitoring treatment effectiveness. They can reveal underlying issues that may not be apparent through physical examination alone.  

Common reasons for conducting a blood test include:

  • Monitoring chronic conditions: Tracking liver, kidney, and muscle function over time.
  • Routine health checks: Assessing general well-being on a yearly basis, or more regularly in performance horses.
  • Illness or infection: Diagnosing underlying diseases or infections.
  • Poor performance or lethargy: Investigating causes of fatigue, anemia, or metabolic imbalances.
  • Pre-purchase examinations: Ensuring a horse is in good health before purchase.

Routine Hematology

Hematology examines the cellular components of blood, more specifically a horse’s red and white blood cells, providing essential information about oxygen transport, immune function, and possible infections or inflammation.

A quick science lesson before we get started –

  • Red blood cells (RBCs), also known as erythrocytes, are the most common type of blood cell and are responsible for delivering oxygen to the body’s tissues via the blood. They contain hemoglobin, an iron-containing protein that binds oxygen in the lungs and transports it throughout the body.
  • White blood cells (WBCs), also known as leukocytes, are an important part of the immune system, helping the body fight infection and other diseases. There are several different types of white blood cells, each with its own specific function.

Here’s a breakdown of key parameters:

PCV (Packed Cell Volume): Measures the percentage of red blood cells in the blood. Normal range: 34-48%. High values may indicate dehydration, stress, or be seen in horses undergoing high-level performance training to a high level, such as racehorses. Low values may suggest anemia, blood loss, or an issue with red blood cell (RBC) production.

Hb (Hemoglobin): The protein in red blood cells that carries oxygen. Normal range: 12.5-17.0 g/dL. High levels may be seen in horses who are dehydrated or undergoing high-level performance training. Low hemoglobin (Hb) often accompanies low packed cell volume (PCV) in anemic horses or is seen with horses that have other problems with oxygen transport.

RBC (Red Blood Cell Count): The number of red blood cells. Normal range: 7-11 x 10^6/µL. Increased red blood cells (RBCs) can be seen in dehydration, stress, or high-level performance training, whereas decreased RBCs again may indicate anemia or other problems with oxygen transport.

MCV (Mean Corpuscular Volume): The average size of red blood cells. Normal range: 36-49 fL. This measure is particularly useful for helping classify different types of anemia in horses. A large average size of red blood cell may indicate regeneration of new, large red blood cells, whereas a small mean shows chronic anemia and iron deficiency.

MCHC (Mean Corpuscular Hemoglobin Concentration): The average concentration of hemoglobin in red blood cells. Normal range: 29-38 g/dL. Aids in anemia classification.

MCH (Mean Corpuscular Hemoglobin): The average amount of hemoglobin per red blood cell. Normal range: 11-17 pg, and also assists in anemia classification.

WBC (White Blood Cell Count): The total number of white blood cells, crucial for immune response. Normal range: 4500-9000/µL. Elevated WBCs suggest infection or inflammation, while decreased WBCs can indicate certain viral infections or suppressed immune function/bone marrow suppression.

Neutrophils (N): A type of white blood cell that is the ‘first responder’ to bacterial infections. Normal range: 55-70%. High values indicate bacterial infection or inflammation, while low values can suggest severe viral infection or immune suppression.

Lymphocytes (L): White blood cells involved in immune response against viruses and other threats and are part of adaptive immunity. Normal range: 30-45%. High values can indicate viral infections or stress, while low values might suggest certain infections or immune suppression.

Monocytes (M): White blood cells that engulf and destroy cellular debris and foreign substances. Normal range: <5%. High values often accompany inflammation or chronic infections. Low levels are unlikely to be a concern.

Eosinophils (E): White blood cells involved in allergic reactions and parasite defense. Normal range: <3%. High values suggest allergies or parasitic infestations. Low levels are unlikely to be a concern.

PLT (Platelets): Essential for blood clotting. No standard range. Low PLTs can lead to bleeding problems, while high PLTs are extremely rare but may increase the risk of blood clots.

ParameterNormal RangeMeaningHigh Values IndicateLow Values Indicate
PCV (Packed Cell Volume)34-48%Measures the proportion of blood made up of red blood cells (RBCs).Dehydration, stress, or high fitness.Anemia, blood loss, or poor RBC production.
Hb (Hemoglobin)12.5-17.0 g/dLOxygen-carrying component in RBCs.Dehydration, high fitness.Anemia, poor oxygen transport.
RBC (Red Blood Cell Count)7-11 x10^6/µLNumber of red blood cells per volume of blood.Dehydration, stress, or high performance training.Anemia, reduced oxygen transport.
MCV (Mean Corpuscular Volume)36-49 fLAverage size of RBCs.Regeneration of new, large RBCs.Chronic anemia, iron deficiency.
MCHC (Mean Corpuscular Hemoglobin Concentration)29-38 g/dLHemoglobin concentration in RBCs.Dehydration or RBC abnormalities.Anemia, iron deficiency.
MCH (Mean Corpuscular Hemoglobin)11-17 pgAverage hemoglobin content per RBC.Regeneration of RBCs.Anemia, poor RBC function.
WBC (White Blood Cell Count)4,500-9,000/µLTotal immune defense cells.Infection, inflammation, stress response.Suppressed immune function, viral infections.
Neutrophils (N%)55-70%First responders to infection.Bacterial infection, stress.Viral infections, immune suppression.
Lymphocytes (L%)30-45%Part of adaptive immunity.Chronic infection, stress.Viral infections, immune suppression.
Monocytes (M%)<5%Involved in chronic inflammation.Chronic inflammation, stress response.Rarely significant.
Eosinophils (E%)<3%Response to allergens or parasites.Parasites, allergic reactions.Rarely significant.
Platelets (PLT)No standard rangeBlood clotting function.Rarely high in horses.Risk of excessive bleeding.

Routine Biochemistry

Biochemistry assesses organ function, muscle health, electrolyte balance, and metabolism. Here are some commonly measured values:

Protein: Total protein level in the blood. Normal range: 55-60 g/l. Elevated levels may indicate dehydration or inflammation, while low levels can suggest liver disease, kidney disease, or protein loss.

Albumin: A major protein in the blood produced by the liver that helps to maintain blood pressure. Normal range: 29-40 g/l. High levels can indicate dehydration. Low albumin can be associated with liver or kidney disease or protein loss.

Globulin: Another type of protein in the blood involved in the immune response. Normal range: 17-42 g/l. High globulin levels may indicate inflammation or infection, while low levels may indicate immune suppression.

Fibrinogen: A protein involved in blood clotting and a marker of inflammation in the body. Normal range: <4 g/l. Elevated levels may suggest inflammation and infections, while low levels are rarely concerning.

AAT (Alpha-1-antitrypsin): A muscle and liver enzyme. Normal range: 183-497 iu/l. Elevated levels suggest muscle damage, liver stress, and inflammation. Low levels are uncommon.

CPK (Creatine Kinase): An enzyme found in muscle and an indicator of muscle function. Normal range: 113-333 iu/l. Elevated levels may indicate muscle damage or overexertion, while low levels may be associated with muscle disease.

AP (Alkaline Phosphatase): An enzyme found in various tissues, including liver and bone. Normal range: <200 iu/l. Elevated levels can suggest liver disease or bone growth. Low levels are rarely significant.

IAP (Isoenzyme of Alkaline Phosphatase): An enzyme specific to intestinal health. Normal range: <20 iu/l. Elevated levels suggest intestinal disease. Low levels are rarely significant.

GLDH (Glutamate Dehydrogenase): An enzyme primarily found in the liver. Normal range: 3-12 iu/l. Elevated levels indicate liver damage, while low levels are rarely significant.

YGT (Gamma-Glutamyl Transferase): An enzyme found in the liver and bile ducts and is used as a marker for liver function. Normal range: <50 iu/l. Elevated levels may suggest liver or bile duct disease or obstructions. Low levels are rarely significant.

Urea: A waste product from protein breakdown by the kidneys. It is a marker of kidney function and protein metabolism. Normal range: 3.5-9.5 mmol/l. Elevated levels may indicate kidney disease or dehydration. Low levels could be seen in horses who have poor diets/protein intake or liver disease.

Creatinine: Another waste product from muscle metabolism, an indicator of kidney filtration and function. Normal range: <167 µmol/l. Elevated levels suggest kidney disease, while low levels could indicate kidney loss.

Bilirubin: A breakdown product of red blood cells. Normal range: 1.3-3.9 µmol/l. Elevated levels may indicate liver disease or red blood cell destruction and are also seen in horses that fast or go a long period without feed. Low levels are rarely significant.

Bile Acids: Produced in the liver and aid in digestion and are a marker of liver function. Normal range: <8 µmol/l. Elevated levels suggest liver disease or bile duct obstruction. Low levels are rarely significant.

SAA (Serum Amyloid A): An acute-phase protein that increases dramatically with inflammation and is therefore a marker of acute inflammation. Normal range: <20 mg/l. Elevated levels indicate acute inflammation and infection. Low levels are rarely significant.

T Bil (Total Bilirubin): The total amount of bilirubin in the blood. Normal range: 13-30 µmol/l. Elevated levels may indicate liver disease or red blood cell destruction.

Iron: Normal range: >119 µg/dL. Iron levels in the blood are essential for good RBC function and are therefore a marker of RBC function and health. Elevated levels may indicate iron overload or liver disease, while low levels suggest iron deficiency anemia.

ParameterNormal RangeMeaningHigh Values IndicateLow Values Indicate
Total Protein55-60 g/LOverall protein levels.Dehydration, inflammation.Poor liver function, protein loss.
Albumin (Alb)29-40 g/LProduced by the liver, maintains blood pressure.Dehydration.Liver disease, protein loss.
Globulin (Glob)17-42 g/LInvolved in immune response.Infection, inflammation.Immune suppression.
Fibrinogen (Fib)<4 g/LClotting protein, inflammation marker.Infection, inflammation.Rarely significant.
AAT (Aspartate Aminotransferase)183-497 IU/LMuscle & liver enzyme.Muscle damage, liver stress.Uncommon concern.
CPK (Creatine Phosphokinase)113-333 IU/LMuscle function indicator.Muscle damage, exertion.Muscle disease.
AP (Alkaline Phosphatase)<200 IU/LLiver and bone enzyme.Liver stress, bone growth.Rarely significant.
IAP (Intestinal Alkaline Phosphatase)<20 IU/LIntestinal health indicator.Intestinal disease.Rarely significant.
GLDH (Glutamate Dehydrogenase)3-12 IU/LLiver-specific enzyme.Liver damage.Rarely significant.
YGT (Gamma-Glutamyl Transferase)<50 IU/LLiver function marker.Liver damage, bile obstruction.Rarely significant.
Urea3.5-9.5 mmol/LKidney function & protein metabolism.Kidney disease, dehydration.Poor protein intake, liver disease.
Creatinine (Creat)<167 µmol/LKidney filtration function.Kidney disease.Muscle loss.
Bilirubin (Total Bilirubin, T Bil)13-30 µmol/LBreakdown product of RBCs.Liver disease, fasting.Rarely significant.
Bile Acids<8 µmol/LLiver function test.Liver disease.Rarely significant.
Serum Amyloid A (SAA)<20 mg/LAcute inflammation marker.Infection, inflammation.Rarely significant.
Iron>119 µg/dLEssential for RBC production.Overload, liver disease.Deficiency, anemia.

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